Studies on .ALPHA.-amino acid ester hydrolase of Xanthomonas citri. II. Substrate specificity of .ALPHA.-amino acid ester hydrolase from Xanthomonas citri.
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چکیده
منابع مشابه
3D Structure Modeling of Alpha-Amino Acid Ester Hydrolase from Xanthomonas rubrilineans
Alpha-amino acid ester hydrolase (EC 3.1.1.43, AEH) is a promising biocatalyst for the production of semi-synthetic β-lactam antibiotics, penicillins and cephalosporins. The AEH gene from Xanthomonas rubrilineans (XrAEH) was recently cloned in this laboratory. The three-dimensional structure of XrAEH was simulated using the homology modeling method for rational design experiments. The analysis ...
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A partially purified preparation of an alpha-amino acid ester hydrolase was obtained from Acetobacter turbidans A.T.C.C. 9325, which catalyses synthesis of 7-(d-alpha-amino-alpha-phenylacetamido)-3-cephem-3-methyl-4- carboxylic acid (cephalexin) from methyl d-alpha-aminophenylacetate and 7-amino-3-deacetoxycephalosporanic acid. The enzyme preparation catalysed both cephalosprin synthesis from 7...
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Acknowledgments. We thank Eimile Dougherty (Cedarville University); Sunhali Sharma and Issac V. Greenhut (Plant Pathology, SWFREC, UF); Peter Newman and Lisa Cushman (Citrus Physiology Laboratory, SWFREC, IFAS, UF, Immokalee, FL), for technical assistance. *Corresponding author; phone: (239) 658-3400; fax: (239) 658-3469; email: [email protected] Effect of Salicylic Acid on Oxidative Metabolism du...
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Copper sprays have been widely used for control of endemic citrus canker caused by Xanthomonas citri subsp. citri in citrus-growing areas for more than 2 decades. Xanthomonas alfalfae subsp. citrumelonis populations were also exposed to frequent sprays of copper for several years as a protective measure against citrus bacterial spot (CBS) in Florida citrus nurseries. Long-term use of these bact...
متن کاملOne Pot Synthesis of Alpha-Aminonitrile and Alpha-Amino Acid From Schiff Bases
Addition of Potassium cyanide to a mixture of Schiff base and lithium perchlorate afforded a-aminonitriles in high yields. Addition of potassium cyanide to a methanolic solution of Schiff base and phosphoric acid afforded a-aminonitriles. When the reaction mixture was refluxed it gave a-amino acids in high yields.
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1980
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.44.1075